Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Human Growth Factor/Immunoglobulin Complexes for Treatment of Myocardial Ischemia-Reperfusion Injury

doi: 10.3389/fbioe.2022.749787

Figure Lengend Snippet: Vascular and myocardial distribution of HGF/IgG complexes 24 h after intracoronary infusion. Note red dots (ALEXA 594-conjugated secondary antibody) on endothelial wall and throughout myocardium in (B) that are not present in vehicle-treated heart (A) . Prior to staining with secondary antisera and DAPI (blue, nuclei), both (A) and (B) were stained with polyclonal primary antisera against 6x His-tag to detect recombinant human HGF. Hearts in (A) and (B) are representative of n = 3 pigs per group that were stained.

Article Snippet: Recombinant rat HGF (Sino Biological US Inc., Wayne, PA, USA) was diluted to a working concentration of 2 μg/ml in sterile PBS.

Techniques: Staining, Recombinant

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Human Growth Factor/Immunoglobulin Complexes for Treatment of Myocardial Ischemia-Reperfusion Injury

doi: 10.3389/fbioe.2022.749787

Figure Lengend Snippet: Multiple administrations of HGF/IgG complexes do not induce HGF- specific auto-antibodies in rats subjected to myocardial ischemia-reperfusion. After MI/R surgery or sham surgery, blood plasma was obtained from rats weekly and assessed for circulating antibodies specific to rat HGF. Animals received three separate treatments: one at reperfusion, one on day 3, and one on day 5 post-MI/R with either vehicle (containing IgG), or HGF/IgG. HGF/IgG-treated animals showed no auto-immune reactivity to HGF. However, weekly addition of human FGF2 to HGF/IgG complexes (positive xenogenic control) induced antibodies to rat HGF, as shown by a mild immune response at weeks 3 and 4. Data represent mean ± SD and were analyzed by repeated measures two-way ANOVA. n = 5 animals, ** p < 0.01.

Article Snippet: Recombinant rat HGF (Sino Biological US Inc., Wayne, PA, USA) was diluted to a working concentration of 2 μg/ml in sterile PBS.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Oleic acid induces A7r5 cell proliferation and migration associated with increased expression of HGF and p-p38

doi: 10.3892/mmr.2021.12123

Figure Lengend Snippet: Effects of OA on the expression of HGF in A7r5 cells. (A) ELISA results at 24 h identified that low concentrations of OA (50 µmol/l) upregulated the level of HGF, but HGF was downregulated at high concentrations (800 µmol/l) of OA. (B) Western blot analysis at 24 h demonstrated that low concentrations of OA (50 µmol/l) increased the expression of HGF, but high concentrations of OA (800 µmol/l) downregulated this expression. (C) Western blot analysis showed that the increased HGF expression at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). (D) Reverse transcription-quantitative PCR analysis at 24 h indicated that the increased expression of HGF at low concentrations of OA (50 µmol/l) was mitigated by PHA665752 (0.1 µmol/l). Data are presented as the mean ± SD (n=3). **P<0.01 and ***P<0.001 vs. 0 µM OA group; ## P<0.01 and ### P<0.001 vs. 50 µmol/l OA group. OA, oleic acid; HGF, hepatocyte growth factor.

Article Snippet: Commercial HGF ELISA kit (cat. no. EK1301) was purchased from Boster Biological Technology.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction